Epogen

Todd Harris


I. Company

A. Background

B. Epogen and Neupogen

II. Epogen

A. Makup

B. Uses

III. Epogen Studies

A. Pediatric patients
1. Safety
2. Efficacy
B. Erythropoietin
1. Kidney
2. Genetically engineered
C. Quality of life
1. Improvements
2. Anemia
D. Severe heart disease
1. High dosage
2. Death

IV. Benefits

A. No guarantee
B. Blood transfusions

C. Kidney transplants

V. Side-effects

A. Stinging

B. Embryo risks

VI. Ethics

A. Cost

B. Availability

VII. Conclusion

Appendix. . . . Personal Experience


Company

Epogen is produced by the company Amgen. Amgen located atThousand Oaks, California, is a global biotechnology company. It discovers, develops, manufactures, and markets human therapeutics based on advanced cellular and molecular biology. Amgen, founded in 1980, is now the largest biotechnology company in the world. It has 4,200 employers around the world and 2,700 of the 4,200 workers, work at the headquarters in Thousand Oaks. Amgen has centers of research in "Boulder, Colorado and Toronto, Canada; clinical research centers in Cambridge, England and Melbourne, Australia; the international distribution center in Louisville, Kentucky, fill and finish facility in Junco, Puerto Rico; and European regional headquarters in Lucerne, Switzerland. Amgen has other international offices that are located in Austria, Australin, Belgium, Canada, China, France, Germany, Italy, Japan, the Netherlands, Hong Kong, Portugal, Spain, and the United Kingdom." (Amgen 1996)

Amgen's first product, Epogen was approved in June 1989 for use in the United States. Neupogen (filgrastim) Amgen's second product, received approval for use in February 1991, is used for preventing infections in cancer patients that receive chemotherapy for bone marrow and peripheral blood progenitor cell transplantation patients and treatment for chronic neutropenia, which is a rare blood disorder. Amgen has research in the areas of hematopoiesis, neurobiology, inflammation/autoimmunity, and soft tissue repair and regeneration. Products from the research, in the four areas mentioned from above, may, sometime in the future, be used in treating conditions characterized by disorders of blood and bone marrow, neurodegenerative diseases such as ALS, Parkinson's, Alzheimer's, or traumatic nerve injury.

Epogen

Epoetin Alfa recombinant Epogen, or Epogen, has 3,000 units of recombinant Epoetin Alfa, 2.5 mg Albumin (Human) in sterile buffered solution (pH 6.9 +/- 0.3) of sodium citrate (5.8mg), sodium chloride (5.8mg), citric acid (0.06mg), in Water for injection.

Its uses are stimulating the body to make red blood cells, treatments of anemia (loss or reduction of red blood cells) associated with chronic renal failure or anemia caused by AZT (zidovudine) in HIV-infected persons. Before Epogen was produced or used, blood transfusions and male hormone therapy was used in treating anemia, which was caused by chronic renal failure.

Epogen Studies

Studies on Epogen are available from Amgen, Food and Drug Administration (FDA), and the Journal of the American Society of Nephrology. One study by Amgen was "to establish the safety and efficacy of EPOGEN in pediatric patients" (Amgen 89), the second study from FDA, was about erythropoietin, which is a protein produced by the kidney and is genetically engineered by scientists, the third study from the Journal of the American Society of Nephrology, was about improvements in the quality of life for kidney dialysis patients, and the last study, also by Amgen, was about how Epogen in dialysis patients who have severe heart disease was receiving high doses of Epogen, which ended up killing the patients instead of helping them, but this is ONLY the patients who are on dialysis with severe heart diseases. The other patients without severe heart diseases are according to the reports okay and have not died because of Epogen.

This study by Amgen was to make sure that Epogen is safe and beneficial for children (pediatric patients). Before the study was initiated, the company (Amgen) had done clinical trials in about 15 children. Once Amgen had received the test results showing that the clinical trials was safe and beneficial, the company then began a nationwide study consisting of about 200 pediatric patients, who have end-stage renal disease and need hemodialysis (the machine), or peritoneal dialysis, which is in a plastic bag.

The dialysis machine takes out the blood from the body and cleans it. The machine does this by diffusing waste products through the tubing that contains a permeable membrane, by a fluid called dialysate. The concentration gradient between the blood and fluid (dialysate) has to be maintained and in order to do that the dialysate has to be continuously replaced. This technique is done three times a week for several hours, this could damage blood cells, and anticoagulants must be added, to avoid possibilities of bleeding problems. On the other hand, Continuous Ambulatory Peritoneal Dialysis (CAPD), does not take as long as the machine, it also does not cause possible damage to the blood cells, but because the catheter has to stay inside the body, there is a high risk for infection. The reason CAPD does not take long to do is because the catheter is inside the peritoneal cavity which has a selectively permeable membrane (peritoneum), this allows substances to move back and forth across the membrane very quickly. CAPD like the machine uses dialysate, but instead of a machine pulling blood out and pumping blood back into the body, the dialysate is put directly into the peritoneal cavity, from a plastic bag (hanging above the abdomen) by mere gravity and then when the patient is ready to discard the waste products (dialysate has been dwelling in the body for at least two hours or more), the patient then puts the plastic bag below the abdomen and opens the roller clamp ( device that pinches off tubing so fluid will not flow out or to allow fluid to flow out at proper time) to allow waste and used dialysate back out and then he/she is ready to put in new dialysate and start the whole process again. This process can be done almost anywhere ( as long as it is clean and sterile) and depending upon the results of the patient's blood tests, weight, and blood pressure the amount of times the process is done during the day varies.

Amgen's study procedures had two parts to it. The first part is randomized and the second part is nonrandomized. The first 12 weeks the patient has a chance of getting Epogen or a placebo ( salt and protein solution, but no Epogen in it). The placebo is neither helpful nor harmful to the patient. During this time, the doctor, the nurse, the patient's parents, and the patient do not know which the patient is receiving, the placebo or Epogen, and Amgen used code numbers for the patient's ID, so the company could not be able to really tell who was getting the placebo or Epogen, anyway. If the patient had the placebo for the first 12 weeks, then he/she would get Epogen for 36 weeks and if he/she was on Epogen for the first 12 weeks, then he/she would get Epogen for 24 weeks. Also, if the red blood cell count gets high too quickly or above the desired level the doctor should then reduce the dose. In the second part of the study everybody knows that the patient is getting the Epogen. The patient will receive Epogen 3 times a week, (Epogen at this time was a single dose in a vile) but can be changed to less or more times during the week in order to obtain the right level. For patients living too far away, family members can be trained to give shots 3 times a week. The shots are rotated right to left in the upper part of the arms and from right to left in the upper part of the legs. Some patients take the shot in the stomach (ouch!). The amount of iron in the blood has to be monitored (in case patient needs more iron, iron pills are prescribed) as well as the blood pressure has to be monitored. Numerous sheets of logs need to be computed to monitor drug administration, and blood pressure, when the log sheets were used up they are sent back to the company Amgen. The study runs tests such as physical examinations every 6 months, chest x-rays, blood samples, culture taken from peritoneal fluid of peritoneal dialysis patient before entering the study, throughout the study patient's diet is nutritionally analyzed by what the patient consumes and how much is consumed, height and weight, head circumference of patients that are 36 months of age or less, tests that determine bone's age and hormone levels, and sexual development taken throughout the study. The testing is for determining the "quality of life" (Amgen 89) and learning ability. Each month blood was drawn to check the red blood cell count, and every so often tests were computed on mental and emotional well being. There were drawing tests to check long term memory, there was a vocabulary test, there were questions to answer about emotional as well as physical state, there was a math test, there were tests for mental reflexes, and also tests for short term memory.

The second study is in the clinical series on erythropoietin was for AIDS patients who are anemic or at the early stages of infection with AIDS virus experience while taking AZT (zudovudine). Through " treatment IND (Investigational New Drug)" (FDA 1991) protocol in June of 1989, the FDA (Food and Drug Administration) permitted erythropoietin, while being investigated for AIDS indication made widely available to patients on AZT. The study involved 297 AIDS patients, who experienced severe anemia from zudovudine (AZT). From the 297 AIDS patients, 118 of them were involved in a placebo-controlled trial, in which patients who had low Epo (erythropoietin) levels resulted in 40% reduction in blood transfusions for Epoetin-treated patients during the three month period. The conclusion to the study was patients with relatively low levels of naturally occurring erythropoietin may benefit from the treatment. Amgen manufactured and did most of the work in developing erythropoientin. The product will be marketed under agreement with Ortho Pharmaceutical Corp. (location: Raritan, N.Y.) who sponsored the clinical trials and treatments for IND (Investigational New Drug) for erythropoietin in AIDS patients. Amgen will be marketing Epoetin Alfa as Epogen and Ortho will be marketing it under the name of Procrit.

The third study was published in the Journal of the Amgen Societyof Nephrology. The study showed genetically engineered protein Epogen(Epoetin Alfa) provides substantial impact on the quality of life in kidney dialysis patients. In the study, patients who receive the epogen treatments attained "significant improvements in vitality, physical functioning, social functioning, mental health, looking after the home, social life, hobbies, and satisfaction with sexual activity." (Journal of the American Society of Nephrology) Many patients receiving dialysis were severely handicapped by the debilitating anemia, found in about 90% of dialysis patients. The study, National Cooperative Recombinant Human Erythropoietin Study, dialysis patients were given standard tests which gaged the many dimensions of functional health and well-being. The results were then measured against the results in groups of patients who had other diseases that had received the same standard tests.
Dialysis patients who not yet received Epogen usually had lower baseline scores than patients with congestive heart failure or chronic depression. After about 99 days of Epogen treatments, their quality of life scores were significantly higher than those who had the other disorders. Vitality was the most affected by the treatment.

The fourth study, was about higher doses of Epogen given to dialysis patients who had severe heart disease, such as congestive heart failure and heart attack. Doctors thought that boosting the red blood cell count of the patients by using more of the Epogen would improve the patient's heart condition, but instead it made their death rates increase. 631 patients out of more than 1,200 dialysis patients with severe heart disease, received Epogen in a dosage that maintained their red blood cell counts at a level commonly accepted during treatment. Of those 631 patients 181 or 28.6% died. In a group 634 patients whose red blood cell counts were boosted by Epogen to a higher level commonly found in people without kidney disease, 221 or 34.8% died. The doctors then heading the study decided Saturday 22, 1996 to end it, after a panel of outside investigators alerted Amgen to the patients deaths two weeks ago. "Dr. Allen Nissenson, professor of medicine at the University of California at Los Angeles and principal investigator of the study said": 'Nothing in these study results suggests that any changes should be made in the treatment of dialysis patients who are receiving the currently approved treatment.'(Amgen 1996)

Benefits

The benefits and side-effects of Epogen are many. Amgen's statement on benefits follows:
No benefit is guaranteed from participating in this study ( Amgen's nationwide study Epogen being tested on pediatric patients). However, Epogen may increase your child's hematocrit ( red blood cell count) and eliminate the need for blood transfusions. Blood transfusions carry the risk of infections and the risk of eliminating certain kidneys for possible transplantation because of the danger of rejection reactions to the kidney. Treatmentwith Epogen has been shown effective in correcting anemia in adults. (Amgen 89)
Also, now the Epogen comes in multidose in a vial, where in the study it was just a single dose in a vial. The advantage to this is that a patient doesn't have to get a vial each month, this avoids having to pay more money out for Epogen. Although shots are a discomfort, the reduction in transfusions is a big advantage.

Side-effects

The potential risks or side-effects are some adults have aches and pains after infusion (of Epogen). If the drug is given under the skin, it may sting or burn, now the medicine has a saline solution added to it, where as before patients had to mix the saline with the Epogen if the Epogen stung.The side-effects a patient may have are high blood pressure, clotting in the device that's used to connect the patient to the dialysis machine, headache, nausea, vomiting, muscle aches, diarrhea, high potassium, a cough may occur, and rare instances of seizures. If the side-effects develop into rapid heartbeat, shortness of breath, hives, itching, or skin rash, tell the doctor right away. Amgen did not see any other side-effects in the 15 pediatric patients from the study. If the patient is pregnant before the study she is not allowed to participate, and if she is pregnant during the study, Epogen is stopped, because of the unknown risks to the embryo. Also, if she decides to become sexually active during the study she must agree to use birth control that the doctor "considers effective and medically acceptable" (Amgen 89), and that the patient "accept the risk that pregnancy could result." (Amgen 89)

Ethics

Shelly White, a nurse a Nephrology Incorporated indicates that Epogen is available to both peritoneal dialysis and hemodialysis patients. Patients on hemodialysis receive the shot while they are receiving dialysis treatment from the machine. A person's insurance will pay for Epogen, but that Medicare eventually, pays for it. Lisa Woodcox of the billing department at Nephrology Incoporated says that the amount of Epogen in the vial given to the patient determines the cost. For example, one vial of 40,000 units costs $250. The cost may also depend upon how much the insurance company will pay. Sometimes insurance will pay 100 percent, but usually most insurances will pay 80 percent.
Amgen says that Epogen was out on the market starting in 1989 all the way up to the present and is still on the market, which means it has been on the market for 7 years now. Also Procrit, which is still Epoetin Alpha just a different name for Epogen, was on the market starting on January of 1991 also up to the present and is still on the market for 5 years now. The Ortho Biotech customer service indicates that Epogen is available to people whose doctor prescribes it to them. Basically through the doctor's prescription to people, that the doctor believes needs it. Also, Epogen is available to dialysis patients in other countries as Erythropoietin Human Recombinant Epoetin Alpha.

Conclusion

From an ethical point of view I would say that the Amgen company did test and research Epogen thoroughly for adults as well as children. The advantage point of Epogen is that it reduces the amount of blood transfusions, reduces the rejection reaction of possible kidney transplants, and reduces the risk of infection. The disadvantage point of Epogen is it is given through injection, which gives a small chance for infection, medicine stings, possiblity of headache, nausea, vomitting, high blood pressure, high potassium, a cough, rapid heartbeat, shortness of breath, itching, hives, or skin rash. Also, if patient is pregnant, there are unkown risks to the embryo. Although there seems to be more side-effects than benefits, the benefits outweigh the side-effects, Epogen studies done by Amgen proves, and therefore is considered safe.


Bibliography

Amgen Incorporated, "A Phase III Double-Blinded, Placebo-Controlled
Study of Epogen (Epoetin alfa) in Pediatric Dialysis Patients (Protocol # EPO-9002) ."
IUPUI INFORMED CONSENT STATEMENT rev. November 1989: pages 1-7.

Amgen Incorporated, "Amgen Inc." Synergistic Designs, Inc., 1996.: Pages 1-2.
World Wide Web http: // www.biospace.com/g/synd/ _c0...hib_script/exhibitors/
AMGENInc.html 10/13/96 14:40:27

"Anemia drug trial suspended as some patients' death rates rise." Nando.net The Associated Press,
1996: Pages 1-2. WorldWideWeb
http://www.nando.net/newsroom/ntn/health/062596/health2_2140.html 10/13/96 14:56:17

Food and Drug Administration. "Epo for AIDs Related Condition." News 01/02/1991:
Pages 1-2. World Wide Web http://www.hivpositive. com/f-DrugAdvisories/11-FDA/
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Friedlander, Ed, "Kidney." January 1996: Pages 1-68.
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"Medication Information Sheet (The Cheshire Medical Center) EPOETIN
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White, Shelly. Personal Interview. November 13, 1996.

Woodcox, Lisa. Personal Interview. November 13, 1996.

Ortho Biotech. Personal Interview. November 13, 1996.


Appendix. . . . Personal Experience

Anytime I am allowed to do a report on a topic of my own choice, I usually do it on medicine that I take or have taken, or on one of my
medical problems. I choose this sort of topic in order to learn more about it and also to inform others about me. This is why I chose Epogen. I take Epogen for anemia, and the reason I am anemic is because I have no kidneys, which make erythropoietin, a protein that stimulates the production of red blood cells. I have no kidneys because my two original kidneys never grew after birth (hypoplastic kidneys). From 8 years old to about 16 years old, I had two kidney transplants. The first transplant lasted for 8 years and the second transplant lasted for 8 months. The explanation for why they didn't last for ever is because my body attacked them and beat them up as if it were fighting infections and so after awhile the kidneys wore out. Also, transplanted organs never last for ever. Now I am on a form of dialysis called continuous ambulatory peritoneal dialysis (CAPD) with no kidneys, which surprises most people, but I have heard of people, who are on dialysis and still have their kidneys, this is because they have a kidney disease and not the same problem that I had.

I started dialysis in April of 1991 when I was 16 years old. A couple of months later, my doctor, Dr. Jerry Bergstein told us about a drug called Epogen and its company Amgen. He told us that Epogen was used in adults, but in order for children to take it, they have to be in a study and monitored very closely. Dr. Bergstein wanted me to be on Epogen and thought that I would benefit from it, so we (my mother and I) decided that I would go ahead and be on Epogen. This meant I had to agree to go on the study, but if for any reason I decided to withdraw from the study, I could do so without any penalties. Also, if the doctor decides that it is best for the patient to stop using Epogen or the study is stopped by Amgen, here again there are no penalties. From the research information and from my own personal experience, I feel that Epogen is an ethical drug to use, as well as a drug that was approved safe by the FDA because of the fact that its benefits outweigh the side- effects.